Journal: PLoS ONE

Article Title: Syntaxin Binding Protein 1 Is Not Required for Allergic Inflammation via IgE-Mediated Mast Cell Activation

doi: 10.1371/journal.pone.0058560

Figure Lengend Snippet: A, Wild type, STXBP1 mutant, or heterozygous mice were identified by PCR using DNA from liver cells. B , After 4–5 weeks of culture, liver-derived mast cells (LMC) were examined by toluidine blue staining to determine mast cell purity. A representative photomicrograph is shown (original magnification = ×100). C , LMCs were sensitized with anti-TNP IgE, stained with FITC-conjugated anti-IgE or anti-c-kit antibodies, and examined by flow cytometry. D, STXBP1 +/+ and STXBP1 −/− LMCs were sensitized with anti-TNP IgE and stimulated with TNP-BSA (10 ng/ml) for various times. Whole cell lysates were analyzed by Western blotting for STXBP1 and Actin. E, Sec1/Munc18 (SM) family gene expression profiles were identified by RT-PCR using mRNA from wild type mouse bone marrow-derived mast cells (BMMC), and wild type (STXBP1 +/+ ) or STXBP1 −/− LMCs, with or without TNP-BSA stimulation. All members of SM family are expressed in wild type BMMCs and LMCs. Similar results were observed in 2 independent experiments.

Article Snippet: FITC-conjugated rat anti-mouse IgE (IgG1), FITC-rat IgG1 and FITC-conjugated rat anti-mouse CD117 (c-kit) were purchased from Cedarlane Laboratories (Burlington, ON, Canada).

Techniques: Mutagenesis, Derivative Assay, Staining, Flow Cytometry, Western Blot, Gene Expression, Reverse Transcription Polymerase Chain Reaction

Journal: Cell reports

Article Title: Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition

doi: 10.1016/j.celrep.2021.109916

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: OT-II transgenic mice (stock # 004194) and CD45.1 mice (stock # 002014) were purchase from Jackson Laboratories and crossed to create OT-II;CD45.1 donor-mice which were confirmed by genotyping according to Jackson lab’s protocol for the OT-II transgenes and blood phenotyping with anti-CD45.1 and anti-CD45.2 antibodies (Tonbo Biosciences, clone A20 and clone 104).

Techniques: Virus, Recombinant, SYBR Green Assay, Cell Isolation, DNA Extraction, Enzyme-linked Immunosorbent Assay, Sequencing, Expressing, Software, Luminex